Oxidative protein modification of soluble N‐ethylmaleimide‐sensitive factor attachment protein receptors

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Non-standard abbreviations: N-ethylmaleimide sensitive factor (NSF) Soluble NSF Attachment protein Receptor (SNARE) Soluble NSF Attachment Proteins (SNAP) Transactivating regulatory protein (Tat) von Willebrand factor (vWF)

Transactivating regulatory protein (Tat) von Willebrand factor (vWF) This article has not been copyedited and formatted. The final version may differ from this version. Abstract N-ethylmaleimide sensitive factor (NSF) plays a critical role in the regulation of exocytosis. NSF regulates exocytosis by interacting with a complex containing SNARE molecules, hydrolyzing ATP, and disassembling the SN...

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Oxidative stress causes plasma protein modification.

We investigated the effect of oxidative systems on plasma proteins using Chloramine-T, a source of free radicals. Plasma specimens from 10 healthy volunteers were treated with 40 mmol/L Chloramine-T (1:1 v/v). Total protein and plasma carbonyl levels were evaluated spectrophotometrically. Identification of plasma proteins modifications was performed by SDS-PAGE, protein and lipid electrophoresi...

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Role of vesicle-associated membrane protein-2, through Q-soluble N-ethylmaleimide-sensitive factor attachment protein receptor/R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor interaction, in the exocytosis of specific and tertiary granules of human neutrophils.

We have examined the role of the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) synaptobrevin-2/vesicle-associated membrane protein (VAMP)-2 in neutrophil exocytosis. VAMP-2, localized in the membranes of specific and gelatinase-containing tertiary granules in resting human neutrophils, resulted translocated to the cell surface following neutrophil activation un...

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ژورنال

عنوان ژورنال: The FASEB Journal

سال: 2013

ISSN: 0892-6638,1530-6860

DOI: 10.1096/fasebj.27.1_supplement.890.7